PRF / CGF Clinical Cases

Classic/Current  PRF/CGF Literature:

Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101:e37–44                                         

Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, Gogly B. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101:e45–e50

Dohan DMChoukroun JDiss ADohan SLDohan AJMouhyi JGogly B. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part III: leukocyte activation: a new feature for platelet concentrates? Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Mar; 101(3):e51-5.

Dohan Ehrenfest DM, de Peppo GM, Doglioli P, Sammartino G. Slow release of growth factors and thrombospondin-1 in Choukroun’s platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies. Growth Factors. 2009; 27:63–69

Dong-Seok Sohn, DDS, PhD • Bingzhen Huang, MD, PhD • Jin Kim, DDS, MS, W. Eric Park, DDS4• Charles C. Park DDS. Utilization of Autologous Concentrated Growth Factors(CGF) Enriched Bone Graft Matrix (Sticky Bone) andCGF-Enriched Fibrin Membrane in Implant Dentistry. JIACD December 2015

He X1, Chen WX2, Ban G1, Wei W1, Zhou J1, Chen WJ1, Li XY1. A New Method to Develop Human Dental Pulp Cells and Platelet-rich Fibrin Complex, J Endod. 2016 Nov;42(11):1633-1640

Jae-Hwan Kim | Su-Mi Woo | Nam-Ki Choi | Won-Jae Kim | Seon-Mi Kim | Ji-Yeon Jung. Effect of Platelet-rich Fibrin on Odontoblastic Differentiation in Human Dental Pulp Cells Exposed to Lipopolysaccharide March 2017

Literature Review on PRF:

Balaram Naik, P Karunakar, M Jayadev, and V Rahul Marshal. Role of Platelet rich fibrin in wound healing: A critical review. J Conserv Dent. 2013 Jul-Aug; 16(4): 284–293.

Sujeet Vinayak Khiste and Ritam Naik Tari. Platelet-Rich fibrin as a Biofuel for Tissue Regeneration. 200 ISRN Biomaterials Volume 2013

Eduardo Borie, Daniel García OlivíIara Augusta OrsiKatia GarletBenjamín WeberVíctor Beltrán andRamón Fuentes. Platelet-rich fibrin application in dentistry: a literature review. Int J Clin Exp Med. 2015; 8(5): 7922–7929.

Case Studies for PRF & Regenerative Endodontics:

AAE Clinical Considerations for a Regenerative Procedure Revised 6-8-16, page 2 – PRF recommended

Shivashankar VY, Johns DA, Vidyanath S, Kumar MR. Platelet Rich Fibrin in the revitalization of tooth with necrotic pulp and open apex. J Conserv Dent. 2012 Oct;15(4):395-8

Navin Mishra, Isha Narang,1 and Neelam Mittal2. Platelet-rich fibrin-mediated revitalization of immature necrotic tooth. Contemp Clin Dent. 2013 Jul-Sep; 4(3): 412–415.

Dexton Antony Johns, Vasundara Yayathi Shivashankar, Shoba Krishnamma, and Manu Johns. Use of photoactivated disinfection and platelet-rich fibrin in regenerative Endodontics. J Conserv Dent. 2014 Sep-Oct; 17(5): 487–490.

Isha Narang, Neelam Mittal, and Navin Mishra. A comparative evaluation of the blood clot, platelet-rich plasma, and platelet-rich fibrin in regeneration of necrotic immature permanent teeth: A clinical study. Contemp Clin Dent. 2015 Jan-Mar; 6(1): 63–68.

Hengameh Bakhtiar | Shahram Esmaeili | Setareh Fakhr Tabatabayi | Mohammad Reza Ellini | Mohammad Hossein Nekoofar | Paul M.H. Dummer. Second-generation Platelet Concentrate (Platelet-rich Fibrin) as a Scaffold in Regenerative Endodontics: A Case Series. March 2017

Case Studies for PRF & Surgical Endodontics:

Toffler M, Toscano N, Holtzclaw D, Corso MD, Dohan Ehrenfest DM. Introducing Choukroun’s platelet rich fibrin (PRF) to the reconstructive surgery milieu. J Implant Clin Adv Dent. 2009;1:21–30.

Simonpieri A, Del Corso M, Vervelle A, Jimbo R, Inchingolo F, Sammartino G, Dohan Ehrenfest DM. Current knowledge and perspectives for the use of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in oral and maxillofacial surgery part 2: Bone graft, implant and reconstructive surgery. Curr Pharm Biotechnol.2012;13:1231–1256. 

Rhythm BainsVivek K. BainsKapil LoombaKavita Verma, and Afreena Nasir. Management of pulpal floor perforation and grade II Furcation involvement using mineral trioxide aggregate and platelet rich fibrin: A clinical report. Contemp Clin Dent. 2012 Sep; S223–S227.

Smita SinghArunendra SinghSourav Singh, and Rashmi Singh. Application of PRF in surgical management of periapical lesions. Natl J Maxillofac Surg. 2013 Jan-Jun; 4(1): 94–99.

Kavitha AnantulaAliveni Annareddy. Platelet-rich fibrin (PRF) as an autologous biomaterial after an endodontic surgery: Case reports. J NTR Univ Health Sci 2016;5:49-54

Pooja Gupta Dudeja, MDS, Krishan Kumar Dudeja, MDS, Arvind Garg, MDS, Dhirendra Srivastava, MDS, Shibani Grover, MDS. Management of a Previously Treated, Calcified, and Dilacerated Maxillary Lateral Incisor: A Combined Nonsurgical/Surgical Approach Assisted by Cone-beam Computed Tomography J Endodo  2016 June;42:6, 984–988

Lee Edward S., Platelet-rich fibrin in endodontics, Endodontic Practice Journal, 2017 Volume 10 Number 1.

Download “Platelet-rich fibrin in endodontics” :    article Clinical-Lee(1)(2)

PRF(or CGF):

Blood draw and centrifuged – PRF clot separated in tube then removed – PRF placed into processing box, then pressed into membranes – Ready to be delivered.

Case 1 – #10 socket preservation using NovaBone, Collagen membrane, CGF membrane.




Case #2 – #13 socket preservation, NovaBone, CGF membrane.





6 month later – CGF sinus bump + Flapless implant placement

Case #3 – #14 mesial buccal root amputation, “sticky” bone allograft, CGF membrane



24   Immediate Post-Op

   1 Month Post Op

  7 Month Post Op

7 Month Post Op

14 month Post Op

Case #4 – #10 socket preservation using allograft + PRF, resorbable collagen membrane, custom fitted PRF membrane (compressed PRF directly into EXT site)

Case #5 – #3 fractured root – Extraction first then later PRF bone graft + PRF membrane


6 months later: Good bone – PRF sinus bump – flapless implant placement

1 month post op: